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Manual of Immunopathological Techniques
Usha MD
Gyanendra Kumar Sonkar PhD
Sangeeta Singh PhD

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Edition 1/e
Publish Year2014
Size 6.75" X 9.50"
Cover TypePaper Back
With CD/DVD No
FormatFour Color
Quick Overview
This manual includes the techniques of basic immunology, autoimmune diseases, tumor immunology, transplant immunology and immunohistochemistry, described by various authors from time-to-time. These techniques have been standardized and modified in our laboratory.

Most of the tests have been standardized in our laboratory, keeping our limited facilities and economic constraints, prevalent at most of Indian Centers in order to make it more useful, easily performed and selectively hassle free. It includes mostly practical aspect of the tests. We hope that this manual will help both medical and nonmedical students to perform tests of basic and transplant immunology, tumor immunology, autoimmune diseases and immunohistochemistry in their laboratory. This manual provides broad overview to different techniques that are commonly used in modern research. Each chapter provides a brief introduction, the procedures and limitations in executing the various procedures along with interpretation of the result. Reagents’ preparation has been well described. The other aim is to provide a better access to the scientific knowledge and to allow the researchers to critically evaluate the results and conclusions. Those working in the field of biological or medical research at any level will definitely benefit from this manual. This manual integrates older as well as newer techniques that will further enhance the learning experience. Therefore, the material provided here is so designed to present clear and concise understanding about various techniques.
Key Features
Manual of Immunopathological Techniques is collection of chapters contributed by experienced immunologists/immunopathologists, all of whom have hands on expertise of techniques they described. The idea of this manual arose from observations that those working in clinical/research field needed at some time or another, to perform an immunological technique applied to their own specific field of research. The existing manuals in market are either too detailed or require background expertise that the user may not necessarily possess. This manual is intended for use in broad clinical/research field including immunology, immunopathology, pathology, biochemistry, animal and veterinary sciences, hematology and genetics of both medical and basic sciences.

This manual has emphasized on both the older techniques, which are still carried out in several laboratories but not available in books and which still yield good results and are cheap and best. It also contains advanced techniques like human leukocyte antigen (HLA) typing, polymerase chain reaction (PCR). fluorescence in situ hybridization (FISH), and flow cytometer, etc. which will help the laboratory people to perform the test in accordance with good laboratory practices (GLP).

In spite of much use of internet for getting one's answer related to different methodologies, this manual will definitely find a place in the laboratories as there is no such book available in the market to our best of knowledge. Each chapter provides readers with a brief introduction about the topic/technique, its principle followed by step-by-step description of methodology, interpretation of the results, colored photographs and lastly the preparations of reagents with points to remember.
Target Audience
Undergraduates and pathologist
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