Manual of Practical Medical Biochemistry has been updated keeping in line with the guidelines and maintaining a clear focus of teaching the first-year medical students, the art of practical medical biochemistry. The revised edition has become necessary in view of the changing trends in the teaching methodology and learning processes. Keeping this in mind, changes have been made throughout this edition, adding details of latest equipments, procedures and laboratory requirements which are mandatory now. Updated Clinical Case studies and Objective Structured Practical Examination (OSPE) and other integrated topics are the highlights of this manual.